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Glass Bottom Dishes are used in the fields of Life Science and Environmental Research, Molecular and Cell Biology,
Cell Culture and Cell Physiology, Biotechnology, Neurology, Pathology, Pharmacology and more.
These sterile tissue culture grade polystyrene glass bottom WillCo Wells Petri dishes with lid are available in two
sizes, 35 x 10mm and 50 x 7mm, with clear or black plastic sidewalls. Through their advanced design features, they provide exceptional image
quality needed for use on inverted microscopes and for applications such as high – and super resolution image analysis, microinjection
and electro-physiology recording of fluorescent-tagged cells.
The WillCo Wells glass bottom Petri dishes use a special formulated adhesive with a low toxicity that is durable and optically
clear. Glass thickness stays within 0.17mm and ra ±0.005mm and permits use of immersion objectives with water, glycerine or oil.
The glass bottom is flush with the microscope stage or heating stage to ensure absolute flatness and optimum heat exchange. The
optical quality of the glass bottom provides a higher effective numerical aperture (NA) producing brighter high resolution images
in all types of microscopy applications.
The 50mm WillCo Wells glass bottom Petri dish features a low rim side wall. The low side wall allows easier access for
micromanipulation/microinjection probes. The WillCo Wells glass bottom petri dish is available in two sizes: 35mm diameter and
50mm diameter. Both sizes are available with clear or black side walls.
The 35mm dish will be compatible with most 35mm heaters and
perfusion adapters.
The 50mm dishes, ‘Series 5030’ and ‘Series 5040’, have a well of respectively 30mm and 40mm, both providing a large growth area. They have a low access angle and an "easy grip" handling.
PELCO® Clear Wall Glass Bottom Dishes
35 x 10mm Clear Wall Glass Bottom Dishes
Available in Blister Packs or Sleeves of 20
50 x 7mm Clear Wall Glass Bottom Dishes
Applications:
- Live Cell Microscopy
- Multi-Photon Laser Scanning Microscopy (MPLSM)
- High Level Laser Fluorescence
- Fluorescence Lifetime Imaging Microscopy (FLIM)
- Fluorescence Resonance Energy Transfer Microscopy (FRETM)
- Fluorescence Redistribution After Photobleaching Microscopy (FRAPM)
- Epifluorescence Microscopy
- Fluorescence Imaging
- Immunofluorescence Microscopy
- Confocal Microscopy
- Confocal Laser Scanning Microscopy (CLSM)
- Phase Contrast Microscopy
- Polarized Light Microscopy
- Differential Interference Contrast
- High Resolution Image Analysis
- Time Lapsed Photography
- Video-Enhanced Microscopy
- Infrared imaging
- Calcium imaging
- Colocalization studies
- Green Fluorescent Protein (GFP)
- Micromanipulation
- Microinjection
- Super resolution imaging
- Nanoscopy imaging
Advantages:
-
Much larger effective range, using an objective lens with a short working distance
-
No birefringence effects because of thin (Class #1.5H / 0.17mm) glass bottom
-
Low rim/side wall of the dish and a larger effective working distance make micromanipulation and microinjection possible
-
Higher effective N.A. make brighter images (in e.g.: fluorescence)
-
Higher effective N.A. make higher resolutions in image analysis
Microscope light passes through the bottom glass in a perpendicular way, glass bottom rests directly on stage
-
Direct heating from the microscope-stage, because the glass is in direct contact (flush) with stage
-
In IVF, no more oocyte/embryo transfer is needed from one plastic dish to another, unless different
media are being used, saving a lot of time (Do not put in incubator!)
-
Easy "perfusion" in the dish for continuous in and outflow of solutions, using "perfusion chamber insert", for life cells.
-
The German glass, borosilicate D263M, is used in microscopy for medical, biological and research work, e.g. in fluorescence microscopy
Fields of Application
Fertility & Infertility
ICSI, spermatid identification, assisted hatching, embryo biopsy, genetics, etc.
(Cell-) Physiology
Patch clamping, ion-imaging, etc.
Pathology
Studies e.g. on the morphology of particles and cells, etc.
Neurology
Axon growth, in and efflux studies of electrolytes, etc.
Cell Biology
Cell-cell communication studies, tissue culture, etc.
Molecular Biology
Studies on genetics (DNA), enzymatic activity, etc.
Pharmacology
Cell research, in and outside the cell on all levels.
Plant Physiology
Root hair growth, micro-parasites on roots, etc.
Cross section view of Black Wall
and Clear Wall Glass Bottom Dishes
Product
Number
Dish
OD
Aperture
ID
Optical Glass OD
Dish
ID
Outside Height
Access Angle
14020
14028
35mm
12mm
34mm
27mm
10mm
36°
14022
14030
35mm
22mm
34mm
27mm
10mm
36°
14024
14032
50mm
30mm
49mm
44mm
7mm
17°
14026
14035
50mm
40mm
49mm
44mm
7mm
17°
PELCO® Clear Wall Glass Bottom Dishes in individual Sterile Blister Packs or Bulk Sterile Sleeves of 20
Prod #
Description
Unit
Price
Order / Quote
Dish: 35mm OD x 10mm High, Glass Area: ø12mm, Access Angle: 37°
14020-20
35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 12mm), Blister Packs
pkg/20
$
132.00
Qty:
14020-120
35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 12mm), Blister Packs
cs/120
647.95
Qty:
14021-20
35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 12mm), Sleeve/20
1 Sleeve
99.50
Qty:
14021-200
35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 12mm), Sleeve/20
cs/10
Sleeves
1007.00
Qty:
14021-500
Glass Bottom Dish Assembly Kit, Clear, 35/12mm
cs/500
1078.00
Qty:
Dish: 35mm OD x 10mm High, Glass Area: ø22mm, Access Angle: 37°
14022-20
35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 22mm), Blister Packs
pkg/20
129.00
Qty:
14022-120
35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 22mm), Blister Packs
cs/120
647.95
Qty:
14023-20
35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 22mm), Sleeve/20
1 Sleeve
99.50
Qty:
14023-200
35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 22mm), Sleeve/20
cs/10
Sleeves
875.95
Qty:
14023-500
Glass Bottom Dish Assembly Kit, Clear, 35/22mm
cs/500
1207.00
Qty:
Dish: 50mm OD x 7mm High, Glass Area: ø30mm, Access Angle: 20°
14024-20
50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 30mm), Blister Packs
pkg/20
128.00
Qty:
14024-120
50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 30mm), Blister Packs
cs/120
647.95
Qty:
14025-20
50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 30mm), Sleeve/20
1 Sleeve
99.50
Qty:
14025-200
50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 30mm), Sleeve/20
cs/10
Sleeves
982.00
Qty:
14025-500
Glass Bottom Dish Assembly Kit, Clear, 50/30mm
cs/500
1078.00
Qty:
Dish: 50mm OD x 7mm High, Glass Area: ø40mm, Access Angle: 20°
14026-20
50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 40mm), Blister Packs
pkg/20
118.95
Qty:
14026-120
50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 40mm), Blister Packs
cs/120
647.95
Qty:
14027-20
50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 40mm), Sleeve/20
1 Sleeve
99.50
Qty:
14027-200
50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 40mm), Sleeve/20
cs/10
Sleeves
982.00
Qty:
14027-500
Glass Bottom Dish Assembly Kit, Clear, 50/40mm
cs/500
1078.00
Qty:
PELCO® Black Wall Glass Bottom Dishes
35 x 10mm Black Wall Glass Bottom Dishes
50 x 7mm Black Wall Glass Bottom Dishes
Applications specific to Black Wall Glass Bottom Dishes use (applications listed above for Clear Wall may also be applicable):
- Live Cell Microscopy
- For low background fluorescent applications
- Confocal Microscopy
- Inverted Microscopy
- High Resolution Image Analysis
- Micromanipulation
- Differential Interference Contrast (HMC)
- Infrared Imaging
Advantages: Are the same as above for Clear Wall Glass Bottom Dishes, PLUS
- The black dish, absorbs more scattering light (Considered debris!).
- The black dish, working in a dark room, makes one see the meniscus of the media much better.
- The black dish, when lid (black) is closed, protects the cells inside completely, from light.
PELCO® Black Wall Glass Bottom Dishes in individual Sterile Blister Packs or Bulk Sterile Sleeves of 20
Prod #
Description
Unit
Price
Order / Quote
Dish: 35mm OD x 10mm High, Glass Area: ø12mm, Access Angle: 37°
14028-20
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Blister Packs
pkg/20
$
123.00
Qty:
14028-120
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Blister Packs
cs/120
647.95
Qty:
14029-20
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Sleeve/20
1 Sleeve
99.50
Qty:
14029-200
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Sleeve/20
cs/10
Sleeves
983.00
Qty:
14029-500
Glass Bottom Dish Assembly Kit, Black, 35/12mm
cs/500
1078.00
Qty:
Dish: 35mm OD x 10mm High, Glass Area: ø22mm, Access Angle: 37°
14030-20
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Blister Packs
pkg/20
131.00
Qty:
14030-120
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Blister Packs
cs/120
647.95
Qty:
14031-20
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Sleeve/20
1 Sleeve
99.50
Qty:
14031-200
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Sleeve/20
cs/10
Sleeves
965.00
Qty:
14031-500
Glass Bottom Dish Assembly Kit, Black, 35/22mm
cs/500
1551.00
Qty:
Dish: 50mm OD x 7mm High, Glass Area: ø30mm, Access Angle: 20°
14032-20
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Blister Packs
pkg/20
131.00
Qty:
14032-120
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Blister Packs
cs/120
670.00
Qty:
14033-20
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Sleeve/20
1 Sleeve
106.00
Qty:
14033-200
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Sleeve/20
cs/10
Sleeves
874.00
Qty:
14033-500
Glass Bottom Dish Assembly Kit, Black, 50/30mm
cs/500
1078.00
Qty:
Dish: 50mm OD x 7mm High, Glass Area: ø40mm, Access Angle: 20°
14035-20
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Blister Packs
pkg/20
132.00
Qty:
14035-120
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Blister Packs
cs/120
647.95
Qty:
14036-20
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Sleeve/20
1 Sleeve
99.50
Qty:
14036-200
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Sleeve/20
cs/10
Sleeves
946.00
Qty:
14036-500
Glass Bottom Dish Assembly Kit, Black, 50/40mm
cs/500
1078.00
Qty:
PELCO® Clear Wall Foil Bottom Dishes
PEN Foil for Microdissection
50 x 7mm Clear Wall
FOIL Bottom Dishes
- Application: Laser Microdissection
- Size: 50 x 7mm
- Bottom: PEN Foil, 2 micron, 4 micron
- Aperture: 30mm
- Vol/μL: 1100μL
- Vents: No
- Sterile R: 5 Years
SALE!
Black Wall Foil Bottom Dishes
PELCO® Clear & Black Wall Foil Bottom Dishes in individual Sterile Blister Packs or Cases of 6 Boxes of 20
Prod #
Description
Unit
Price
Order / Quote
Dish: 50mm OD x 7mm High, Foil Area: ø30mm, Access Angle: 20°
14037-20
50 x 7mm, PELCO® Clear Wall Foil Bottom Dishes (4μm Foil 30mm), Blister Packs
pkg/20
$
196.00
Qty:
14037-120
50 x 7mm, PELCO® Clear Wall Foil Bottom Dishes (4μm Foil 30mm), Blister Packs
cs/120
1168.00
Qty:
Glass Bottom Dish Assembly Kit
Build your own Glass Bottom Dishes and save 55% or more compared to the pre-assembled product.
This easy do-it-yourself kit is available in two dish sizes and four bottom aperture sizes, with clear - or black walls and lids.
Each kit includes:
- 500 dishes and lids
- 500 top quality (SCHOTT D263M) round glass coverslips of corresponding size
- 500 double-sided adhesive rings (3M™)
- a set of tweezers and,
- a pair of protective latex gloves
Special two-part Assembly Device (available separately) made of high quality anodized aluminum serves to safely, precisely, easily, and quickly position the adhesive ring and the glass coverslip bottom and evenly distribute the gluing pressure.
The assembly routine is very simple and clean, it does not involve messy glues or the need for precise aim and motor skill. A ring of matching diameter carrying the specially formulated adhesive is peeled off its backing and mounted onto the blue part of the Assembly Device. The dish bottom, guided precisely by the top protrusion of the Device, is then pressed firmly on top of the ring. This achieves transfer of the Double Sided Adhesive (DSA) ring onto the dish bottom. Now take the second silicon liner of from the ‘DSA’ ring and the now sticky dish is pressed against the corresponding glass coverslip, which has been secured within the special slot in the red part of the assembly device. After four hours at room temperature to allow the bonding to complete, the newly made glass bottom dish is ready to use. Detailed assembly instructions are included with each WillCo Wells kit.
Each kit is supplied with #1.5H thickness and ra 0.005mm coverslip glass bottoms, unless specified as #1 when placing the order (custom).
Special two-part Assembly Device (available separately) made of high quality anodized aluminum serves to safely, precisely, easily, and quickly position the adhesive ring and the glass coverslip bottom and evenly distribute the gluing pressure.
Prod #
Description
Unit
Price
Order / Quote
Clear Wall Glass Bottom Dish Assembly Kit
14021-500
Glass Bottom Dish Assembly Kit, Clear, 35/12mm
cs/500
$
1078.00
Qty:
14023-500
Glass Bottom Dish Assembly Kit, Clear, 35/22mm
cs/500
1207.00
Qty:
14025-500
Glass Bottom Dish Assembly Kit, Clear, 50/30mm
cs/500
1078.00
Qty:
14027-500
Glass Bottom Dish Assembly Kit, Clear, 50/40mm
cs/500
1078.00
Qty:
Black Wall Glass Bottom Dish Assembly Kit
14029-500
Glass Bottom Dish Assembly Kit, Black, 35/12mm
cs/500
1078.00
Qty:
14031-500
Glass Bottom Dish Assembly Kit, Black, 35/22mm
cs/500
1551.00
Qty:
14033-500
Glass Bottom Dish Assembly Kit, Black, 50/30mm
cs/500
1078.00
Qty:
14036-500
Glass Bottom Dish Assembly Kit, Black, 50/40mm
cs/500
1078.00
Qty:
Assembly Device for Glass Bottom Dish Kit
The Assembly Device is a set of two high quality tools precision-machined from anodized aluminum. It serves to precisely position the adhesive ring and the glass coverslip bottom and evenly distribute the gluing pressure. A separate Assembly Device is offered for each bottom aperture size: 12mm and 22mm Devices for the 35mm dish size, larger-sized 30mm and 40mm devices for the 50mm dish size.
Prod #
Description
Unit
Price
Order / Quote
14021-0
Assembly Device for Glass Bottom Dish Kit, 12mm
each
$
227.00
Qty:
14023-0
Assembly Device for Glass Bottom Dish Kit, 22mm
each
227.00
Qty:
14025-0
Assembly Device for Glass Bottom Dish Kit, 30mm
each
248.00
Qty:
14027-0
Assembly Device for Glass Bottom Dish Kit, 40mm
each
248.00
Qty: